GSM1541450: H3K27me3 ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
T-47D, H3K27me3 ChIP
cell line
T-47D
cell type
breast cancer cell line
chip antibody
H3K27me3 (Cell Signaling 9733S)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to ChIP-Seq library preparation kit (NEB) and sequenced with the Illumina HiSeq 2000 analyzer at Mayo Clinic.