Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T-47D, H3K27Ac ChIP
cell line
T-47D
cell type
breast cancer cell line
chip antibody
H3K27ac (Abcam ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to ChIP-Seq library preparation kit (NEB) and sequenced with the Illumina HiSeq 2000 analyzer at Mayo Clinic.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
22078279
Reads aligned (%)
98.8
Duplicates removed (%)
1.5
Number of peaks
1441 (qval < 1E-05)

hg19

Number of total reads
22078279
Reads aligned (%)
98.1
Duplicates removed (%)
1.9
Number of peaks
1345 (qval < 1E-05)

Base call quality data from DBCLS SRA