Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC UCLA2
NA
NA

Attributes by original data submitter

Sample

source_name
UCLA2 sorted hPGCLCs
cell type
aggregates
chip antibody
none (input)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq of hESCs, iMeLCs, day 4 aggregates was performed using anti-TFAP2C antibodies and libraries were constructed with Ovation Ultralow Systems V2 (NuGEN, 0344). ChIP-seq of sorted day4 hPGCLCs using anti-H3K27ac antibodies was performed using Low Cell ChIP-Seq Kit (ACTIVE MOTIF, 53804).

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
67987098
Reads aligned (%)
97.2
Duplicates removed (%)
14.0
Number of peaks
1323 (qval < 1E-05)

hg19

Number of total reads
67987098
Reads aligned (%)
96.5
Duplicates removed (%)
15.2
Number of peaks
1401 (qval < 1E-05)

Base call quality data from DBCLS SRA