ChIP-seq of hESCs, iMeLCs, day 4 aggregates was performed using anti-TFAP2C antibodies and libraries were constructed with Ovation Ultralow Systems V2 (NuGEN, 0344). ChIP-seq of sorted day4 hPGCLCs using anti-H3K27ac antibodies was performed using Low Cell ChIP-Seq Kit (ACTIVE MOTIF, 53804).