Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TFAP2C

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC UCLA2
NA
NA

Attributes by original data submitter

Sample

source_name
UCLA2 hPGCLC aggregates
cell type
aggregates
chip antibody
TFAP2C
antibody source
Santa Cruz, catalog number: sc-8977, lot G1112

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq of hESCs, iMeLCs, day 4 aggregates was performed using anti-TFAP2C antibodies and libraries were constructed with Ovation Ultralow Systems V2 (NuGEN, 0344). ChIP-seq of sorted day4 hPGCLCs using anti-H3K27ac antibodies was performed using Low Cell ChIP-Seq Kit (ACTIVE MOTIF, 53804).

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
59126560
Reads aligned (%)
36.0
Duplicates removed (%)
56.4
Number of peaks
17520 (qval < 1E-05)

hg38

Number of total reads
59126560
Reads aligned (%)
37.4
Duplicates removed (%)
55.2
Number of peaks
17585 (qval < 1E-05)

Base call quality data from DBCLS SRA