Antigen

Antigen Class

TFs and others

Antigen

TFAP2C

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC UCLA1

NA

NA

Sample

source_name

UCLA1 iMeLC

cell type

iMeLC

chip antibody

TFAP2C

antibody source

Santa Cruz, catalog number: sc-8977, lot G1112

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq of hESCs, iMeLCs, day 4 aggregates was performed using anti-TFAP2C antibodies and libraries were constructed with Ovation Ultralow Systems V2 (NuGEN, 0344). ChIP-seq of sorted day4 hPGCLCs using anti-H3K27ac antibodies was performed using Low Cell ChIP-Seq Kit (ACTIVE MOTIF, 53804).

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

116530564

Reads aligned (%)

95.2

Duplicates removed (%)

42.8

Number of peaks

46350 (qval < 1E-05)

hg19

Number of total reads

116530564

Reads aligned (%)

94.9

Duplicates removed (%)

43.4

Number of peaks

46155 (qval < 1E-05)