Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Uterus
Cell type
Ishikawa
Primary Tissue
Uterus
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Endometrioid adenocarcinoma cell line
cell line
Ishikawa
chip antibody
Santa Cruz ERalpha (HC-20)
treatment
1 hr 10nM E2 and 72 hours non-targeting siRNA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature. Crosslinking was stopped with 125mM Glycine and cells were washed and scraped in cold PBS. IP was done as described in Reddy et al 2009 using Santa Cruz ERα (HC-20) or Millipore ERα (06-935) TruSeq library construction as described in Reddy et al 2009

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
27574233
Reads aligned (%)
75.3
Duplicates removed (%)
22.0
Number of peaks
29726 (qval < 1E-05)

hg38

Number of total reads
27574233
Reads aligned (%)
75.8
Duplicates removed (%)
21.8
Number of peaks
29786 (qval < 1E-05)

Base call quality data from DBCLS SRA