Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MED1

Cell type

Cell type Class
Adipocyte
Cell type
Adipose-Derived Mesenchymal Stem Cells
NA
NA

Attributes by original data submitter

Sample

source_name
human adipose-derived stem cells
cell line
hMADS-3
cell type
human adipose-derived stem cells
chip antibody
MED1 (M-255; sc-8998; Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed in mature hMADS adipocytes essentially as previously described (Siersbæk et al. 2012, MCB, 32: 3452-3463). For cross-linking of chromatin, 0.2 mM disuccinimidyl glutarate (DSG) was applied (Proteochem, Denver, CO) for 45 min followed by cross-linking using 1% formaldehyde for 10 min. ChIP-seq libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014 Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq. Methods in Enzymology 2014, Vol. 537, pp. 261-279).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg19

Number of total reads
12804523
Reads aligned (%)
78.4
Duplicates removed (%)
15.3
Number of peaks
17662 (qval < 1E-05)

hg38

Number of total reads
12804523
Reads aligned (%)
79.9
Duplicates removed (%)
14.2
Number of peaks
17719 (qval < 1E-05)

Base call quality data from DBCLS SRA