Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
ZR-75-1
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
Human Breast Cancer Cells
gender
female
cell line
ZR751
cell type
Luminal
antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Phenol/chlorophrom extraction was used for gDNA isolation. Standard Illumina protocols are followed. ChIP-seq: Bioo Scientific NEXTflex kit is used (Catalog: 514120, 48 reactions) ChIP-seq: Libraries were prepared according to the standard Illumina protocol and sequenced with the HiSeq2500 system. Single end sequencing was carried out to obtain around 25-150 million (M), 100bp long reads per sample.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
106034729
Reads aligned (%)
99.0
Duplicates removed (%)
32.4
Number of peaks
2874 (qval < 1E-05)

hg19

Number of total reads
106034729
Reads aligned (%)
98.1
Duplicates removed (%)
34.3
Number of peaks
1323 (qval < 1E-05)

Base call quality data from DBCLS SRA