GSM1537291: H3K27me3-MDAMB436 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Breast
Cell type
MDA-MB-436
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Human Breast Cancer Cells
gender
female
cell line
MDAMB436
cell type
Basal-Like Carcinoma
antibody
Millipore; ab07449
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Phenol/chlorophrom extraction was used for gDNA isolation. Standard Illumina protocols are followed. ChIP-seq: Bioo Scientific NEXTflex kit is used (Catalog: 514120, 48 reactions) ChIP-seq: Libraries were prepared according to the standard Illumina protocol and sequenced with the HiSeq2500 system. Single end sequencing was carried out to obtain around 25-150 million (M), 100bp long reads per sample.