Antigen

Antigen Class

TFs and others

Antigen

HIF1A

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562 cells

cell type

erythroid leukemia

treatment

1% oxygen

treatment length

72 hours

antibody

Rabbit polyclonal to HIF-1 alpha

antibody catalog number

ab2185 (Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Fixed chromatin was sheared for HIF-1alpha ChIP. ChIPed DNA was reverse-crosslinked and purified for sequencing Library was prepared with standard Illumina protocol by the University of Chicago Sequencing Core

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

65419831

Reads aligned (%)

98.5

Duplicates removed (%)

39.4

Number of peaks

2131 (qval < 1E-05)

hg19

Number of total reads

65419831

Reads aligned (%)

97.5

Duplicates removed (%)

41.3

Number of peaks

1988 (qval < 1E-05)