Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HIF1A

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562 cells
cell type
erythroid leukemia
treatment
1% oxygen
treatment length
72 hours
antibody
Rabbit polyclonal to HIF-1 alpha
antibody catalog number
ab2185 (Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixed chromatin was sheared for HIF-1alpha ChIP. ChIPed DNA was reverse-crosslinked and purified for sequencing Library was prepared with standard Illumina protocol by the University of Chicago Sequencing Core

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
134054995
Reads aligned (%)
37.6
Duplicates removed (%)
61.3
Number of peaks
2301 (qval < 1E-05)

hg38

Number of total reads
134054995
Reads aligned (%)
39.4
Duplicates removed (%)
59.7
Number of peaks
2676 (qval < 1E-05)

Base call quality data from DBCLS SRA