GSM1535700: HNF4a2 IP DOX TCF4 rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TCF7L2
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HNF4a2 Line
cell line
HCT116
cell type
colon cancer
passages
20-26
treatment
0.3 µg/mL DOX
length of treatment
24 hours
antibody
anti-TCF7L2 (TCF4) (Millipore; cat# 6H5-3)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Formaldehyde cross-linking and chromatin immunoprecipiation was performed as described in Hwang-Verslues et al., 2008 (Mol Endo 22:78-90) and Li AG et al., 2007 (Mol. Cell 28:408-421) with some modification. Illumina-compatible NEXTflex ChIPseq Kit (Bioo Scientific) was used to generate libraries from purified ChIP DNA for sequencing in the Illumina's HiSeq2000. Illumina HiSeq2000 was used to sequence the libraries (50bp, single-end)