Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Hematopoietic Stem Cells
MeSH Description
Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Attributes by original data submitter

Sample

source_name
ckit and sca1 positive cells
cell type
Hematopoietic stem and progenitor cells from mouse bone marrow
chip antibody
IgG

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ckit and sca1 positive cells were isolated from the bone marrow of Mx1 Cre:Usp16loxp/loxp mice. DNA was sheared into approximately 200bp fragments by sonication. Usp16 bound DNA was immunoprecipitated by standard protocols. Libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 1000

mm10

Number of total reads
30102288
Reads aligned (%)
84.2
Duplicates removed (%)
11.5
Number of peaks
565 (qval < 1E-05)

mm9

Number of total reads
30102288
Reads aligned (%)
99.4
Duplicates removed (%)
0.0
Number of peaks
1 (qval < 1E-05)

Base call quality data from DBCLS SRA