Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXP1

Cell type

Cell type Class
Neural
Cell type
OF4155
NA
NA

Attributes by original data submitter

Sample

source_name
FOXP1_IP
cell line
OF4155
cell type
Neural progenitor cells
Sex
female
chip antibody
mouse monoclonal anti-FLAG M2
chip antibody vendor
Sigma-Aldrich

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
PROTOCOL1: All RNA samples were examined for quantity and quality by NanoDrop and Bioanalyzer (Agilent). Samples were randomized for RNA extractions and sequencing. 2ug of total RNA were treated with Ribo-zero Removal Kit according to the manufacturer’s instructions to remove ribosomal RNA. PROTOCOL2: DNA was purified using Qiagen MinElute Columns and quantified using a Qubit Fluorometer according to the manufacturer’s instructions. PROTOCOL3: All RNA samples were examined for quantity and quality by NanoDrop and Bioanalyzer (Agilent). Samples were randomized for RNA extractions and sequencing. 2ug of total RNA were treated with Ribo-zero Removal Kit according to the manufacturer’s instructions to remove ribosomal RNA. Libraries were prepared using an Illumina TruSeq kit according to the manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
52903589
Reads aligned (%)
37.2
Duplicates removed (%)
40.7
Number of peaks
1673 (qval < 1E-05)

hg38

Number of total reads
52903589
Reads aligned (%)
39.9
Duplicates removed (%)
37.6
Number of peaks
2013 (qval < 1E-05)

Base call quality data from DBCLS SRA