Antigen

Antigen Class

TFs and others

Antigen

Spi1

Cell type

Cell type Class

Blood

Cell type

Dendritic Cells

MeSH Description

ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Sample

source_name

Sample_MC_05_cDC_pu1

cell type

moDC

tissue

Bone marrow

strain

C57BL/6

chip antibody

Pu.1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixated, lyzed and nuclei were sonicated. Sonicated nuclei were mixed with protein G magnetic dynabeads coupled to target antibbody. Libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

12011516

Reads aligned (%)

97.6

Duplicates removed (%)

11.7

Number of peaks

42771 (qval < 1E-05)

mm9

Number of total reads

12011516

Reads aligned (%)

97.4

Duplicates removed (%)

11.8

Number of peaks

42755 (qval < 1E-05)