Antigen

Antigen Class

TFs and others

Antigen

Irf7

Cell type

Cell type Class

Blood

Cell type

Myeloid Cells

MeSH Description

The classes of BONE MARROW-derived blood cells in the monocytic series (MONOCYTES and their precursors) and granulocytic series (GRANULOCYTES and their precursors).

Sample

source_name

GM-CSF treated myeloid cells

cell type

Myeloid cells

tissue

Bone marrow

strain

C57BL/6

chip antibody

Irf7

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixated, lyzed and nuclei were sonicated. Sonicated nuclei were mixed with protein G magnetic dynabeads coupled to target antibbody. Libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

7262949

Reads aligned (%)

86.2

Duplicates removed (%)

13.2

Number of peaks

735 (qval < 1E-05)

mm9

Number of total reads

7262949

Reads aligned (%)

86.0

Duplicates removed (%)

13.4

Number of peaks

748 (qval < 1E-05)