Antigen

Antigen Class

TFs and others

Antigen

Runx1

Cell type

Cell type Class

Blood

Cell type

EML

NA

NA

Sample

source_name

EML cells

cell type

EML

strain

C57BL/6

chip antibody

Rabbit polyclonal anti-RUNX1 (non-commercial antibody)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and transcription factor-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

31929519

Reads aligned (%)

85.9

Duplicates removed (%)

13.8

Number of peaks

5773 (qval < 1E-05)

mm9

Number of total reads

31929519

Reads aligned (%)

85.8

Duplicates removed (%)

13.8

Number of peaks

5975 (qval < 1E-05)