Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NUP98

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293FT cell line expressing NUP98-FLAG
cell line
HEK293FT cell line expressing NUP98-FLAG
tag
FLAG
cell type
embryonal kidney
chip antibody
Monoclonal ANTI-FLAG® M2 (SIGMA ALDRICH, F3165, lot-SLBG5311 )

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with the antibody. Libraries were constructed using the ChIP-seq sample preparation kit (Illumina, San Diego, California, USA)

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg19

Number of total reads
103543117
Reads aligned (%)
77.0
Duplicates removed (%)
17.4
Number of peaks
1516 (qval < 1E-05)

hg38

Number of total reads
103543117
Reads aligned (%)
78.9
Duplicates removed (%)
16.1
Number of peaks
1422 (qval < 1E-05)

Base call quality data from DBCLS SRA