Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived ectodermal cells
NA
NA

Attributes by original data submitter

Sample

source_name
H9-ectoderm_p53_ IFI16sh1865
cell line
human embryonic stem cell H9
cell type
ectoderm
gender
female
treatment
IFI16 knockdown
antibody
p53 (Santa Cruz, sc-126)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
1x10^7 cells, fragmentation by sonicator, p53-DNA complexes were enriched with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Preparation Kit (IP-202-1024).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
51113245
Reads aligned (%)
96.3
Duplicates removed (%)
50.0
Number of peaks
1457 (qval < 1E-05)

hg38

Number of total reads
51113245
Reads aligned (%)
98.4
Duplicates removed (%)
49.5
Number of peaks
2351 (qval < 1E-05)

Base call quality data from DBCLS SRA