Antigen

Antigen Class

TFs and others

Antigen

BRD4

Cell type

Cell type Class

Neural

Cell type

90-8TL

NA

NA

Sample

source_name

MPNST cells 90-8TL_DMSO

cell line

MPNST cell line 90-8TL

treated with

DMSO for 24hrs

chip antibody

BRD4

chip antibody vendor

Bethyl

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIPed following the Agilent Mammalian ChIP-on-chip protocol. Cells were cross- linked for 15 min at room temperature by the addition of one-tenth of the volume of 11% formaldehyde solution to the growth media. NEBNext ChIP Master Mix Kit for Illumina following manufacturers instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

21809477

Reads aligned (%)

90.4

Duplicates removed (%)

33.3

Number of peaks

30190 (qval < 1E-05)

hg19

Number of total reads

21809477

Reads aligned (%)

90.2

Duplicates removed (%)

33.6

Number of peaks

30233 (qval < 1E-05)