customized from Beijing B&M Biotech (Beijing, China)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with KDM3A or p-KDM3A antibody.The sonicated DNA fragments were purified as Input control. The DNA fragments were end-repaired, adenylated,ligated to adaptors and PCR-amplified for 18 cycles. The PCR products corresponding to bp 250-450 were gel-purified, quantified and stored at -80°C until use for sequencing