Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KDM3A

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
Jurkat cell line
cell line
Jurkat
cell type
Acute T Cell Leukemia T lymphocyte
treatment
37℃ 1h
chip antibody
KDM3A
antibody manufacturer
Millipore Biotech (Billerica, MA, USA)
antibody catalog number
09-823

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with KDM3A or p-KDM3A antibody.The sonicated DNA fragments were purified as Input control. The DNA fragments were end-repaired, adenylated,ligated to adaptors and PCR-amplified for 18 cycles. The PCR products corresponding to bp 250-450 were gel-purified, quantified and stored at -80°C until use for sequencing

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
24562294
Reads aligned (%)
67.0
Duplicates removed (%)
9.7
Number of peaks
14103 (qval < 1E-05)

hg38

Number of total reads
24562294
Reads aligned (%)
68.2
Duplicates removed (%)
8.7
Number of peaks
10896 (qval < 1E-05)

Base call quality data from DBCLS SRA