Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Germinal center B-cells
NA
NA

Attributes by original data submitter

Sample

source_name
GC B cells
cell type
Germinal Center B cells purified from spleen
strain
129SVE(129S6)
chip antibody
input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq: Cell Lysates were sonicated and histone-DNA complexes were isolated with anti H3K27Ac antibody. ChIP-Seq: Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Preparation Kit. The libraries were sequenced on Illumina HiSeq2500.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
186688844
Reads aligned (%)
97.7
Duplicates removed (%)
20.4
Number of peaks
810 (qval < 1E-05)

mm9

Number of total reads
186688844
Reads aligned (%)
97.3
Duplicates removed (%)
20.2
Number of peaks
1052 (qval < 1E-05)

Base call quality data from DBCLS SRA