GSM1524337: H69 YAP Santa Cruz; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
YAP1
Cell type
Cell type Class
Lung
Cell type
NCI-H69
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Small Cell
Attributes by original data submitter
Sample
source_name
H69 human cell line
cell line
H69
chip antibody
YAP Santa Cruz
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 10 min and cross-linking was quenched by addition of 0.125 M glycine. Cells were then harvested in ChIP buffer and sonicated with a Bioruptor UCD-300 to obtain fragments around 250 bp in size. Precipitated DNA was recovered by purification with a QIAquick PCR purification kit (Qiagen) Libraries for sequencing were obtained using a ChIP-Seq DNA sample prep kit (Illumina) according to the manufacturer's reccomendations