Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TCF21

Cell type

Cell type Class
Cardiovascular
Cell type
Coronary artery smooth muscle
NA
NA

Attributes by original data submitter

Sample

source_name
human coronary artery smooth muscle cells
antibody
TCF21 (HPA013189)
cell type
smooth muscle cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysis by 0.5% SDS and the lysates were clarified from sonicated cells and protein-DNA complexes were isolated with TCF21 antibody (HPA013189). Libraries were prepared with KAPA Hyper Prep kit (KK8502) per standrad instruction.

Sequencing Platform

instrument_model
HiSeq X Ten

hg19

Number of total reads
515297944
Reads aligned (%)
24.2
Duplicates removed (%)
87.3
Number of peaks
44952 (qval < 1E-05)

hg38

Number of total reads
515297944
Reads aligned (%)
25.8
Duplicates removed (%)
86.6
Number of peaks
49668 (qval < 1E-05)

Base call quality data from DBCLS SRA