GSM4209477: Input for AsPC1 IRF1 empty IFNgamma ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pancreas
Cell type
AsPC-1
Primary Tissue
Pancreas
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
AsPC1_empty vector and cultured overnight in the presence of 0.2ng/ul IFNgamma
cell line
AsPC1
cell type
pancreatic ductal adenocarcinoma (PDA) cell line
genotype/variation
lentivirally transduced with empty vector and cultured overnight in the presence of 0.2ng/ul IFNgamma
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After cross-link, cells were incubated with cell lysis buffer (10mM Tris pH8.0, 10mM NaCl, 0.2% NP-40) and then resuspended and sonicated in nuclei lysis buffer (50mM Tris pH8.0, 10mM EDTA, 1%SDS) for 15mins, followed by ChIP Library was constructed using Illumina TruSeq ChIP Sample Prep kit following manufacture's protocol. Briefly, ChIP DNA was end repaired, followed by A tailing and size selection (250-500 bp) by gel electrophoresis. 15 PCR cycles were used for final library amplification and the library was analyzed on Bioanalyzer using high sensitivity DNA chip (Agilent) on Bio-analyzer.