Cells were seeded in 100-mm dishes at 2 × 106 cells/dish then cross-linked with 1% formaldehyde and immunoprecipitation was performed overnight with Nrf2-specific antibody (Santa Cruz Biotechnology) and IgG as control following the instructions with the immunoprecipitation kit (Upperstate, Shanghai, China) The sequencing and generation of short DNA reads of immunoprecipitated samples were carried out at Genenergy Inc. (Shanghai, China) using an Illumina Hiseq 2000.