Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFE2L2

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549 NSCLC cells
cell type
lung epithelial adenocarcinoma cell line
passages
15-18
chip antibody
Nrf2-specific antibody (Santa Cruz Biotechnology)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were seeded in 100-mm dishes at 2 × 106 cells/dish then cross-linked with 1% formaldehyde and immunoprecipitation was performed overnight with Nrf2-specific antibody (Santa Cruz Biotechnology) and IgG as control following the instructions with the immunoprecipitation kit (Upperstate, Shanghai, China) The sequencing and generation of short DNA reads of immunoprecipitated samples were carried out at Genenergy Inc. (Shanghai, China) using an Illumina Hiseq 2000.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
30723388
Reads aligned (%)
6.2
Duplicates removed (%)
7.4
Number of peaks
750 (qval < 1E-05)

hg38

Number of total reads
30723388
Reads aligned (%)
6.3
Duplicates removed (%)
6.8
Number of peaks
727 (qval < 1E-05)

Base call quality data from DBCLS SRA