GSM4202000: ZF-Input-Pooled; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
MV4;11 cells
treatment
DMSO/100nM/200nM THZ531
antibody
N/A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crossed-linked with formaldehylde solution. Then nuclear extracts were isolated, and sonicated. Chromatin bound to Pol II were isolated using RNA polymerase II, clone CTD4H8, mouse monoclonal antibody. ChIP-Seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645). Fragments between 200 and 500 base pairs were selected using a Pippin Prep 1.5% agarose cassette (Sage Science, Beverly MA US), pooled and 75 single-end base pair reads were sequenced using the Illumina NEXTseq 500. NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645)