Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TAL1

Cell type

Cell type Class
Blood
Cell type
T-ALL
NA
NA

Attributes by original data submitter

Sample

source_name
T-ALL
cell type
MOLT3
chip antibody
TAL1
antibody catalog
sc-12984
young_id
20140725_2889
system
Blood
tal1 enhancer status
WT/MOLT3 WT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified. Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. 

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
45516545
Reads aligned (%)
73.3
Duplicates removed (%)
24.9
Number of peaks
32874 (qval < 1E-05)

hg38

Number of total reads
45516545
Reads aligned (%)
74.9
Duplicates removed (%)
23.9
Number of peaks
33029 (qval < 1E-05)

Base call quality data from DBCLS SRA