GSM4198778: spike-in Input NTC-DMS; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
CA46
Primary Tissue
Blood
Tissue Diagnosis
Malignant Lymphoma - Burkitts Type
Attributes by original data submitter
Sample
source_name
cell line: Human B lymphocyte CA46 cells; spike-in reference organism: Drosophila melanogaster; spike-in cell line: S2
cell type
Human B lymphocyte cell line
cell line
CA46
genotype/variation
non-targeting control
treatment
exposed to PBS for 20h
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The CA46 cells' ChIP-seq experiments with spike-in control was referred to the manufacturer's instruction (Active Motif). In this experiment, the DNA fragments were sonicated between 200bp to 500bp. For H3K4me3 ChIP and a Drosophila-specific antibody (Active Motif 61686), 1.5 X 107 CA46 cells were used. As to RNA-seq, total RNA was extracted using TRIZOL Reagent (Life technologies) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100.Qualified total RNA was further purified by RNeasy micro kit and RNase-Free DNase Set. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample ChIP-sequencing libraries were prepared according to generated using NEBNext® Ultra™ IIDNA Library Prep Kit (#E7645). RNA-sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.