Antigen

Antigen Class

TFs and others

Antigen

CHD8

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC derived neural cells

NA

NA

Sample

source_name

Neurons

cell type

ES-derived neuron

differentiation time

3 weeks

genotype/variation

WT

chip-antibody

Anti CHD8 : Rabbit-Bethyl lab-A301-224A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Extract protocol for ChIP-seq, RNA-seq and ATAC-seq are according to "method" section in the manuscript. RNA and ChIP libraries were prepared for sequencing using standard Illumina protocols. ATAC : we followed the cited protocol in the method section: Chen, X. et al. Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity. Nature communications 9, 4590, doi:10.1038/s41467-018-07115-y (2018).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

35073811

Reads aligned (%)

63.6

Duplicates removed (%)

5.4

Number of peaks

853 (qval < 1E-05)

hg19

Number of total reads

35073811

Reads aligned (%)

63.0

Duplicates removed (%)

6.4

Number of peaks

500 (qval < 1E-05)