Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EGR1

Cell type

Cell type Class
Uterus
Cell type
Endometrial stromal cells
NA
NA

Attributes by original data submitter

Sample

source_name
t-HESC cell line
cell line
t-HESC
antibody
EGR1 (Cell Signaling 4154)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and DNA complexes were isolated with antibody Cell Signaling 4154 ChIP and Input DNAs were prepared for amplification by converting overhangs into phosphorylated blunt ends and adding an adenine to the 3'-ends. Illumina genomic adapters were ligated and the sample was size-fractionated (200-300 bp) on a 2% agarose gel. After a final PCR amplification step (18 cycles), the resulting DNA libraries were quantified and sequenced on NextSeq 500.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
36962153
Reads aligned (%)
45.9
Duplicates removed (%)
51.2
Number of peaks
5036 (qval < 1E-05)

hg38

Number of total reads
36962153
Reads aligned (%)
47.5
Duplicates removed (%)
49.7
Number of peaks
5301 (qval < 1E-05)

Base call quality data from DBCLS SRA