Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Embryo
Cell type
Embryos
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic cells
strain
CG21 egl-30(tg26) I; him-5(e1490) V
chip antibody
H3K27ac (Abcam: ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Staged embryos designated for ChIP-seq analysis were cross-linked with a 2% formadehyde solution (diluted in 500 mL M9 buffer) for 30 minutes, followed by the addition of 125 mM glycine to quench the reaction, and two final washes in PBS + protease inhibitors. Extracts were prepared from synchronized egl-30(tg26) embryos by standard methods described previously (Reichsteiner et al. 2010, Ercan et al. 2007). Approximately 2 mg of extract from each embryo stage was immunoprecipitated with 2 ug of commercially available antibodies previously cited in C. elegans chromatin studies Extracted DNA was prepared into ChIP-seq libraries using the NEBNext Ultra DNA library preparation kit for Illumina (New England Biolabs) and was sequenced on the HiSeq 2500 platform

Sequencing Platform

instrument_model
Illumina HiSeq 2500

ce11

Number of total reads
21068003
Reads aligned (%)
0.0
Duplicates removed (%)
11.4
Number of peaks
6104 (qval < 1E-05)

ce10

Number of total reads
21068003
Reads aligned (%)
0.0
Duplicates removed (%)
11.4
Number of peaks
6104 (qval < 1E-05)

Base call quality data from DBCLS SRA