GSM1518912: BRG1siSOX10 CHIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMARCA4
Cell type
Cell type Class
Epidermis
Cell type
501A
Primary Tissue
Skin
Tissue Diagnosis
Melanoma
Attributes by original data submitter
Sample
source_name
501Mel
cell line
501Mel
cell type
melanoma
chip antibody
BRG1 : Abcam (clone EPNCIR111A, ab110641)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
BRG1 ChIP experiments were performed on native Mnase-digested chromatin isolated from 501Mel cells (Kasinathan et al., 2014). SOX10 and 3HA-MITF ChIP experiments were performed on 0,4% PFA-fixed chromatin isolated from 501Mel and Cl8 cells respectively according to standard protocols as previously described (Strub et al., 2011). Up to 60 ug of chromatin was subjected to immunoprecipation with a specific antibody. The libraries were sequenced on the Illumina Hiseq 2500 as single-end 50 base reads following Illumina’s instructions