GSM4182509: ChIP Opa late rep1; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
opa
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
source_name
ChIP_IP
age at harvest
4h embryo collection (mid nc14 to gastrulation)
genotype
wt
chip antibody
In-house anti-Opa antibody
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500.