Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
opa

Cell type

Cell type Class
Unclassified
Cell type
Unclassified
NA
NA

Attributes by original data submitter

Sample

source_name
ChIP_IP
age at harvest
3h embryo collection (nc13 to mid nc14)
genotype
wt
chip antibody
In-house anti-Opa antibody

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
16748185
Reads aligned (%)
68.6
Duplicates removed (%)
18.3
Number of peaks
9699 (qval < 1E-05)

dm6

Number of total reads
16748185
Reads aligned (%)
67.0
Duplicates removed (%)
20.0
Number of peaks
9765 (qval < 1E-05)

Base call quality data from DBCLS SRA