immortalized, developmentally arrested megakaryocyte-erythroid progenitors (MEPs) derived from in vitro differentiation of murine Gata1-ES cells
transduced with
MIGR1-GATA1fl-HA
timepoint
bipotential progenitors at 42 hrs post transduction
chip antibody
anti-HA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and HA-GATA1 or HA-GATA1s DNA complexes were isolated with anti-HA antibody. Libraries were prepared using the protocol as outlined for Illumina's TruSeq ChIP Sample Prep Kit ( IP-202-1012), except that the libraries were size selected using Agencourt SPRIselect beads for an average size of ~300-325bp prior to PCR amplification. Library quality was assessed using the Aglient Bioanalzyer 2100 and libraries were sequenced on the Illumina HiSeq2000.