Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
mouse bone marrow derived macrophages
treatment
dex
gender
male
age
10 week old
strain
C57BL/6
antibody
input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
10M Cells per condition were fixed in 1% formaldehyde for 10min at RT, lysed and sonicated. GR-DNA complexes were then isolated from soluble chromatin using BuGR2 monoclonal antibody (Pierce), crosslinks reversed at 65 degrees and DNA cleaned up using Minielute columns(Qiagen) Multiplex libraries were prepared by Edinburgh Genomics using Illumina standard protocols and reagents. Libraries were sequenced on the Hiseq2500 following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
6975882
Reads aligned (%)
99.0
Duplicates removed (%)
4.7
Number of peaks
131 (qval < 1E-05)

mm9

Number of total reads
6975882
Reads aligned (%)
98.9
Duplicates removed (%)
4.7
Number of peaks
99 (qval < 1E-05)

Base call quality data from DBCLS SRA