Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
O-GlcNAc

Cell type

Cell type Class
Adipocyte
Cell type
Adipocytes
MeSH Description
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.

Attributes by original data submitter

Sample

source_name
human in vitro differentiated adipocyte day 11 treated with 10 mM of glutamine during differentiation
cell type
in vitro differentiated adipocyte
passages
15-18
treatment
High glutamine
chip antibody
O-Linked N-Acetylglucosamine (RL2) (a2739)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared using NEBNext ChIP-seq library Prep master mix set (New England Biolabs, USA).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
8848157
Reads aligned (%)
94.5
Duplicates removed (%)
30.4
Number of peaks
3335 (qval < 1E-05)

hg19

Number of total reads
8848157
Reads aligned (%)
93.6
Duplicates removed (%)
31.1
Number of peaks
3295 (qval < 1E-05)

Base call quality data from DBCLS SRA