Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Sample_DMSO_ET_Control_Input
cell line
MCF-7
cell line
Breast Cancer Cells
genotype
Control
sgguide
sgNT
chip-antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The fixed cells were lysed in SDS buffer and the chromatin was sheared by Covaris sonication. The sheared chromatin was incubated with the aforementioned antibodies and protein G-Dynabeads. The samples underwent decrosslinking, RNase and proteinase K treatment. DNA fragments were eluted using AMP Pure beads, library was prepared and samples were subjected to high-throughput sequencing using HiSeq 2000 platform (Illumina). 

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
25809523
Reads aligned (%)
97.5
Duplicates removed (%)
4.6
Number of peaks
654 (qval < 1E-05)

hg19

Number of total reads
25809523
Reads aligned (%)
96.6
Duplicates removed (%)
5.9
Number of peaks
923 (qval < 1E-05)

Base call quality data from DBCLS SRA