Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

Mamary gland epithelial cell

cell type

Breast adenocarcinoma

gender

female

chip antibody

HA tag (abcam: ab9110)

cell line

MCF-7

agent

Veh

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

MCF-7 chromatin was sonicated and PAX2 bond genomic regions were isolated by using HA tag antibody. Libraries were prepared by Norwegian sequencing centre and CRUK genomics core facility

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

43670712

Reads aligned (%)

97.0

Duplicates removed (%)

45.5

Number of peaks

746 (qval < 1E-05)

hg19

Number of total reads

43670712

Reads aligned (%)

96.4

Duplicates removed (%)

46.6

Number of peaks

685 (qval < 1E-05)