Antigen

Antigen Class

TFs and others

Antigen

KDM1A

Cell type

Cell type Class

Digestive tract

Cell type

SW 480

Primary Tissue

unresolved

Tissue Diagnosis

unresolved

Sample

source_name

SW480 Cells

genotype

wildtype

chip antibody

LSD1 (abcam, ab17721)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were trypsonized prior to histone ChIP-seq (iDeal ChIP-seq Kit for Histones, Diagenode: C01010051) or scraped prior to transcription factor ChIP-seq (iDeal ChIP-seq kit for Transcription Factors, Diagenode: C01010055). Libraries were generated for sequencing using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) per manufacturer's protocol.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

44271834

Reads aligned (%)

99.2

Duplicates removed (%)

7.1

Number of peaks

1724 (qval < 1E-05)

hg19

Number of total reads

44271834

Reads aligned (%)

98.3

Duplicates removed (%)

7.2

Number of peaks

1660 (qval < 1E-05)