Naïve resting B cells from splenocytes of 8 week old male C57BL6 mice were incubated with anti-CD43 beads (Miltenyi) and the negatively selected population isolated and confirmed as 95% CD19+ by flow cytometry (FACS Calibur). RNA libraries were prepared with the NuGEN Ovation RNA-Seq v2 library preparation kit, which amplifies both mRNA and non-polyadenylated transcripts. The resulting cDNA was fractionated by sonication and prepared using the Illumina TruSeq library preparation kit which was then multiplexed across single-end, 100bp (RNA), 50bp (ChIP and miRNA), lanes of an Illumina HiSeq2000 sequencer using the Illumina pipeline RTA version 1.12.4.2 and de-multiplexing with CASAVA v1.8.2. ChIP-seq was performed at NIH using an Illumina TruSeq ChIP library kit according to the manufacturer’s protocol followed by cluster generation with a TruSeq Cluster generation kit v5. ChIP libraries were sequenced on an Illumina Genome Analyzer (GA-II) with sequencing performed for 42 cycles using the Illumina RTA version1.8.