GSM1508945: sf7761 H3K4me3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Neural
Cell type
SF7761
NA
NA
Attributes by original data submitter
Sample
source_name
whole cell
cell type
SF7761 cell line
cell cycle stage
asynchronization
chip antibody
H3K4me3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed the same way as ChIP assay, except that DNA was recovered from the protein G complex using elution buffer (10 mM Tris at pH 8.0, 10 mM EDTA at pH 8.0, 1% SDS, 150 mM NaCL, 5 mM DTT). DNA was subsequently purified using Qiagen Mini Elute PCR purification kit. Total RNA was extracted using the miRNeasy Mini Kit (Qiagen #217004). The ChIP DNA libraries were prepared with the Ovation Ultralow DR Multiplex system (NuGEN). RNA-seq libraries were prepared with ovation RNA-seq system v2 kit (NuGEN).