Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SuUR

Cell type

Cell type Class
Embryo
Cell type
2h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
pooled stage 12 and 13 egg chambers
strain
OrR
chip antibody
SUUR (made in-house)
developmental stage
pooled stage 12 and 13
tissue
egg chamber

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Egg chambers were dounced and sonicated using a Biorupto 300 (Diagenode) Libraries were constrcted using the NEB Next ChIP seq kit (Part # E6200S) according to manufactures recommendations with indexing primers for multiplexing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
21233132
Reads aligned (%)
32.8
Duplicates removed (%)
44.0
Number of peaks
1323 (qval < 1E-05)

dm6

Number of total reads
21233132
Reads aligned (%)
31.1
Duplicates removed (%)
46.6
Number of peaks
2083 (qval < 1E-05)

Base call quality data from DBCLS SRA