Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
2h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
pooled stage 12 and 13 egg chambers
strain
OrR
chip antibody
NA
developmental stage
pooled stage 12 and 13
tissue
egg chamber

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Egg chambers were dounced and sonicated using a Biorupto 300 (Diagenode) Libraries were constrcted using the NEB Next ChIP seq kit (Part # E6200S) according to manufactures recommendations with indexing primers for multiplexing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
24399240
Reads aligned (%)
92.5
Duplicates removed (%)
14.1
Number of peaks
372 (qval < 1E-05)

dm3

Number of total reads
24399240
Reads aligned (%)
92.5
Duplicates removed (%)
13.3
Number of peaks
381 (qval < 1E-05)

Base call quality data from DBCLS SRA