Antigen

Antigen Class

TFs and others

Antigen

SuUR

Cell type

Cell type Class

Adult

Cell type

Egg chamber

NA

NA

Sample

source_name

stage 10 egg chambers from OrR

strain

OrR

chip antibody

SUUR (made in-house)

developmental stage

stage 10

tissue

egg chamber

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Egg chambers were dounced and sonicated using a Biorupto 300 (Diagenode) Libraries were constrcted using the NEB Next ChIP seq kit (Part # E6200S) according to manufactures recommendations with indexing primers for multiplexing

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

23681642

Reads aligned (%)

67.7

Duplicates removed (%)

52.1

Number of peaks

2286 (qval < 1E-05)

dm3

Number of total reads

23681642

Reads aligned (%)

68.2

Duplicates removed (%)

48.6

Number of peaks

2212 (qval < 1E-05)