Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Neuro-2a

Tissue

Brain

Cell Type

Neuroblast

Disease

Neuroblastoma

Sample

source_name

Neuro2a cells transfected with a FLAG-tag expressing vector

strain

C57BL/6

cell type

Neuro2a

treatment

transfected with FLAG-tag control construct

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysates were sonicated and Lhx5-bound DNA isolated with an anti-FLAG antibody. Cellular protein and RNA was digested and the DNA was purified Libraries were constructed with the NEB CHIPseq Master Mix Prep Kit for Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

36976968

Reads aligned (%)

98.0

Duplicates removed (%)

12.7

Number of peaks

504 (qval < 1E-05)

mm9

Number of total reads

36976968

Reads aligned (%)

97.8

Duplicates removed (%)

12.7

Number of peaks

586 (qval < 1E-05)