Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
KBM-7
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
H3K27ac_HiChIP_MED14-dTAG_2h_DMSO
cell line
KBM7
cell type
chronic myeloid leukemia cell line
genotype/variation
MED14-dTAG
treatment
2h DMSO
chip antibody
H3K27ac (Abcam ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin contacts were generated and pulldown performed as previously described (PMID: 27643841). Library construction using Illumina Tn5.

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg38

Number of total reads
175984719
Reads aligned (%)
87.9
Duplicates removed (%)
10.0
Number of peaks
43464 (qval < 1E-05)

hg19

Number of total reads
175984719
Reads aligned (%)
87.7
Duplicates removed (%)
10.1
Number of peaks
42839 (qval < 1E-05)

Base call quality data from DBCLS SRA