Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PPP1R10

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
U2OS MYC-ER cells
antibody
pPNUTS (see Methods)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Rxseq sample preparation was performed as previously described (Herold et al., 2019). Samples were spiked with 10% NIH3T3 cells. Libraries were prepared as previously described (Herold et al., 2019).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
12657947
Reads aligned (%)
45.0
Duplicates removed (%)
54.7
Number of peaks
1845 (qval < 1E-05)

hg38

Number of total reads
12657947
Reads aligned (%)
46.7
Duplicates removed (%)
53.1
Number of peaks
1886 (qval < 1E-05)

Base call quality data from DBCLS SRA